TCRT October 2012

category image Volume 11
No.5 (409-527)
October 2012
ISSN 1533-0338
Electroporation

BNNT-Mediated Irreversible Electroporation: Its Potential on Cancer Cells (459-465)

Irreversible lethal electroporation (IRE) is a new non-thermal ablation modality that uses short pulses of high amplitude static electric fields (up 1000 V/cm) to create irreversible pores in the cell membrane, thus, causing cell death. Recently, IRE has emerged as a promising clinical modality for cancer disease treatment. Here, we investigated the responses of tumour human HeLa cells when subjected to IRE in the presence of BNNTs. These consist of tiny tubes of B and N atoms (arranged in hexagons) with diameters ranging from a 1 to 3 nanometres and lengths < 2 μm. BNNTs have attracted wide attention because of their unique electrical properties. We speculate that BNNTs, when interacting with cells exposed to static electrical fields, amplify locally the electric field, leading to cell death. In this work, electroporation assays were performed with a commercial electroporator using the cell- specific protocol suggested by the supplier (exponential decay wave, time constant 20 ms) with the specific aim to compare IRE in absence and in presence of BNNTs. We observed that BNNTs have the capacity to decrease substantially the voltage required for IRE. When cells were pulsed at 800 V/cm, we observed a 2,2-fold reduction in cell survival in the presence of BNNTs compared to controls. We conclude that the death of the tumour cells exposed to IRE is strongly enhanced in the presence of BNNTs, indicating their potential therapeutic application.

Key words: Boron nitride nanotubes; Irreversible electroporation.

DOI: 10.7785/tcrt.2012.500258

V. Raffa, Ph.D.1,2*†
C. Riggio, M.Sc.1†
M. W. Smith, Ph.D.3
K. C. Jordan, Ph.D.4
W. Cao, Ph.D.5
A. Cuschieri, FRS.1

1Life Science Institute, Scuola Superiore Sant’Anna, Pisa, Italy
2Department of Biology, University of Pisa, Pisa, Italy
3NASA Langley Research Centre, Hampton, VA, USA
4Jefferson Lab, Jefferson National Accelerator Facility, Newport News, VA, USA
5Old Dominion University, Newport News, VA, USA
†These authors equally contributing.

v.raffa@sssup.it

This article can be cited as:
Raffa, V. Riggio, C. Smith, M.W. Jordan, K.C. Cao, W. Cuschieri, A. BNNT-Mediated Irreversible Electroporation: Its Potential on Cancer Cells. Technol Cancer Res Treat. 11, 459-465 (2012)

This article can be accessed at PubMed:
http://www.ncbi.nlm.nih.gov/pubmed/22475064

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