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Transrectal Ultrasound-Integrated Spectral Optical Tomography of Hypoxic Progression of a Regressing Tumor in a Canine Prostate (519-531)

The objective of this study was to evaluate if transrectal optical tomography implemented at three wavelength bands for spectral detection could monitor changes of the hemoglobin oxygen saturation (StO2) in addition to those of the total hemoglobin concentration ([HbT]) in lesions of a canine prostate, including an induced tumor modeling canine prostate cancer. Near-infrared (NIR) optical tomography was integrated with ultrasound (US) for transrectal imaging. Multi-spectral detection at 705 nm, 785 nm and 808 nm rendered measurements of [HbT] and StO2. Canine transmissible venereal tumor (TVT) cells were injected into the right lobe of a dog’s prostate gland, which had a pre-existing cyst in the left lobe. Longitudinal assessments of the prostate were performed weekly over a 63-day duration by NIR imaging concurrent with grey-scale and Doppler US. Ultrasonography revealed a bi-lobular tumor-mass regressing from day-49 to day-63. At day-49 this tumor-mass developed a hypoxic core that became larger and more intense by day-56 and expanded further by day-63. The tumor-mass presented a strong hyper-[HbT] feature on day-56 that was inconsistent with US-visualized blood flow. Histology confirmed two necrotic TVT foci within this tumor-mass. The cyst appeared to have a large anoxic-like interior that was greater in size than its ultrasonographically delineated lesion, and a weak lesional elevation of [HbT]. On day-56, the cyst presented a strong hyper-[HbT] feature consistent with US-resolved blood flow. Histology revealed acute and chronic hemorrhage in the periphery of the cyst. The NIR imaging features of two other TVT nodules and a metastatic lymph node were evaluated retrospectively. Transrectal US-integrated spectral optical tomography seems to enable longitudinal monitoring of intra-lesional oxygenation dynamics in addition to the hemoglobin content of lesions in the canine prostate.

Key words: Prostate cancer; Hypoxia; Optical tomography; Canine transmissible venereal tumor.





This article can be cited as:
Jiang, Z., Piao, D., Bartels, K.E., Holyoak, G.R., Ritchey, J.W., Ownby, C.L., Rock, K., Slobodov, G. Transrectal Ultrasound-Integrated Spectral Optical Tomography of Hypoxic Progression of a Regressing Tumor in a Canine Prostate Technol Cancer Res Treat. 10, 519-531 (2011). DOI: 10.7785/tcrt.2012.500219

References

1. Jemal, A., Siegel. R., Xu. J., Ward. E. Cancer statistics, 2010. CA Cancer J Clin 60, 277-300 (2010).
2. Powers, W. E., Tolmach, L. J. A multicomponent x-ray survival curve for mouse lymphosarcoma cells irradiated in vivo. Nature 197, 710-711 (1963). [Crossref]
3. Shweiki, D., Itin, A., Soffer, D., Keshet, E. Vascular endothelial growth factor induced by hypoxia may mediate hypoxia-initiated angiogenesis. Nature 359, 843-845 (1992). [Crossref]
4. Kunz, M., Hartmann, A., Flory, E., Toksoy, A., Koczan, D., Thiesen, H. J., Mukaida, N., Neumann, M., Rapp, U. R., Bröcker, E. B., Gillitzer, R. Anoxia-induced up-regulation of interleukin-8 in human malignant melanoma. A potential mechanism for high tumor aggressiveness. Am J Pathol 155, 753-763 (1999).
5. Höckel, M., Schlenger, K., Höckel, S., Aral, B., Schäffer, U., Vaupel, P. Tumor hypoxia in pelvic recurrences of cervical cancer. Int J Cancer 79, 365-369 (1998). [Crossref]
6. Sundfør, K., Lyng, H., Rofstad, E. K. Tumour hypoxia and vascular density as predictors of metastasis in squamous cell carcinoma of the uterine cervix. Br J Cancer 78, 822-827 (1998).
7. Movsas, B., Chapman, J. D., Horwitz, E. M., Pinover, W. H., Greenberg, R. E., Hanlon, A. L., Iyer, R., Hanks, G. E. Hypoxic regions exist in human prostate carcinoma. Urology 53, 11-18 (1999). [Crossref]
8. Yu, G., Durduran, T., Zhou, C., Zhu, T. C., Finlay, J. C., Busch, T. M., Malkowicz, S. B., Hahn, S. M., Yodh, A. G. Real-time in situ monitoring of human prostate photodynamic therapy with diffuse light. Photochem Photobiol 82, 1279-1284 (2006). [Crossref]
9. Leith, J. T., Quaranto, L., Padfield, G., Michelson, S., Hercbergs, A. Radiobiological studies of PC-3 and DU-145 human prostate cancer cells: x-ray sensitivity in vitro and hypoxic fractions of xenografted tumors in vivo. Int J Radiat Oncol Biol Phys 25, 283-287 (1993).
10. Vukanovic, J., Isaacs, J. T. Human prostatic cancer cells are sensitive to programmed (apoptotic) death induced by the antiangiogenic agent linomide. Cancer Res 55, 3517-3520 (1995).
11. Tatum, J. L., Kelloff, G. J., Gillies, R. J., Arbeit, J. M., Brown, J. M., Chao, K. S., Chapman, J. D., Eckelman, W. C., Fyles, A. W., Giaccia, A. J., Hill, R. P., Koch, C. J., Krishna, M. C., Krohn, K. A., Lewis, J. S., Mason, R. P., Melillo, G., Padhani, A. R., Powis, G., Rajendran, J. G., Reba, R., Robinson, S. P., Semenza, G. L., Swartz, H. M., Vaupel, P., Yang, D., Croft, B., Hoffman, J., Liu, G., Stone, H., Sullivan, D. Hypoxia: importance in tumor biology, noninvasive measurement by imaging, and value of its measurement in the management of cancer therapy. Int J Radiat Biol 82, 699-757 (2006). Review. [Crossref]
12. Janssen, H. L., Haustermans, K. M., Balm, A. J., Begg, A. C. Hypoxia in head and neck cancer: how much, how important? Head Neck 27, 622-638 (2005). Review. [Crossref]
13. Movsas, B., Chapman, J. D., Hanlon, A. L., Horwitz, E. M., Pinover, W. H., Greenberg, R. E., Stobbe, C., Hanks, G. E. Hypoxia in human prostate carcinoma: an Eppendorf PO2 study. Am J Clin Oncol 24, 458-461 (2001). [Crossref]
14. Jöbsis, F. F. Noninvasive, infrared monitoring of cerebral and myocardial oxygen sufficiency and circulatory parameters. Science 198, 1264-1267 (1977).
15. Tromberg, B. J., Pogue, B. W., Paulsen, K. D., Yodh, A. G., Boas, D. A., Cerussi, A. E. Assessing the future of diffuse optical imaging technologies for breast cancer management. Med Phys 35, 2443-2451 (2008). [Crossref]
16. Zhu, Q., Tannenbaum, S., Kurtzman, S. H. Optical tomography with ultrasound localization for breast cancer diagnosis and treatment monitoring. Surg Oncol Clin N Am 16, 307-321 (2007). Review. [Crossref]
17. Gulsen, G., Birgul, O., Unlu, M. B., Shafiiha, R., Nalcioglu, O. Combined diffuse optical tomography (DOT) and MRI system for cancer imaging in small animals. Technol Cancer Res Treat 5, 351-363 (2006).
18. Pu, Y., Wang, W. B., Tang, G. C., Zeng, F., Achilefu, S., Vitenson, J. H., Sawczuk, I., Peters, S., Lombardo, J. M., Alfano, R. R. Spectral polarization imaging of human prostate cancer tissue using a near- infrared receptor-targeted contrast agent. Technol Cancer Res Treat 4, 429-436 (2005). [Crossref]
19. Cho, H., Ackerstaff, E., Carlin, S., Lupu, M. E., Wang, Y., Rizwan, A., O’Donoghue, J., Ling, C. C., Humm, J. L., Zanzonico, P. B., Koutcher, J. A. Noninvasive multimodality imaging of the tumor microenvironment: registered dynamic magnetic resonance imaging and positron emission tomography studies of a preclinical tumor model of tumor hypoxia. Neoplasia 11, 247-259 (2009).
20. Jiang, Z., Holyoak, G. R., Bartels, K. E., Ritchey, J. W., Xu, G., Bunting, C. F., Slobodov, G., Piao, D. In vivo trans-rectal ultrasound coupled near-infrared optical tomography of a transmissible venereal tumor model in the canine pelvic canal. J Biomed. Opt Lett 14, 030506-1-030506-3 (2009). [Crossref]
21. Jiang, Z., Piao, D., Holyoak, G. R., Ritchey, J. W., Bartels, K. E., Slobodov, G., Bunting, C. F., Krasinski, J. S. Trans-rectal ultrasound-coupled spectral optical tomography of total hemoglobin concentration enhances assessment of the laterality and progression of a transmissible venereal tumor in canine prostate. Urology 77, 237-242 (2011).
22. Zhu, Q. Optical tomography with ultrasound localization: initial clinical results and technical challenges. Technol Cancer Res Treat 4, 235-244 (2005). [Crossref]
23. Prier, J. E., Brodey, R. S. Canine neoplasi. A prototype for human cancer study. Bull World Health Organ, 29, 331-344 (1963). Review.
24. Hill, D. L., Yang, T. J., Wachtel, A. Canine transmissible venereal sarcoma: tumor cell and infiltrating leukocyte ultrastructure at different growth stages. Vet Pathol 21, 39-45 (1984).
25. Fenton, M. A., Yang, T. J. Role of humoral immunity in progressive and regressive and metastatic growth of the canine transmissible venereal sarcoma. Oncology 45, 210-213 (1988).
26. Santos, F. G. A., Vasconcelos, A. C., Nunes, J. E. S., Cassali, G. D., Paixão, T. A., Martins, A. S., Silva, S. S., Martins, R. F., Moro, L. Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases. Arq Bras Med Vet Zootec 60, 607-612 (2008). [Crossref]
27. Pérez, J., Day, M. J., Mozos, E. Immunohistochemical study of the local inflammatory infiltrate in spontaneous canine transmissible venereal tumour at different stages of growth. Vet Immunol Immunopathol 64, 133-147 (1998). [Crossref]
28. Corlu, A., Durduran, T., Choe, R., Schweiger, M., Hillman, E. M. C., Arridge, S. R., Yodh, A. G. Uniqueness and wavelength optimization in continuous-wave multispectral diffuse optical tomography. Opt Lett 28, 2339-2341 (2003).
29. Xu, G., Piao, D., Bunting, C. F., Dehghani, H. Direct-current-based image reconstruction versus direct-current included or excluded frequency-domain reconstruction in diffuse optical tomography. Appl Opt 49, 3059-3070 (2010). [Crossref]
30. Li, A., Zhang, Q., Culver, J. P., Miller, E. L., Boas, D. A. Reconstructing chromosphere concentration images directly by continuous-wave diffuse optical tomography. Opt Lett 29, 256-258 (2004). [Crossref]
31. Hayes, M. D., Vanzant, E. S., Stombaugh, T. S., Gates, R. S. Comparison of bovine blood absorption coefficients to human curves. Livestock Environment VIII Proceedings, 981-5 (2008).
32. Forlani, L., Ioppolo, C., Antonini, E. Calorimetric studies of oxyhemoglobin dissociation. I. Reaction of sodium dithionite with oxygen. J Inorg Biochem 20, 147-155 (1984).
33. Nak, D., Nak, Y., Cangul, I. T., Tuna, B. A clinico-pathological study on the effect of vincristine on transmissible venereal tumour in dogs. J Vet Med A Physiol Pathol Clin Med 52, 366-370 (2005). [Crossref]
34. Kanick, S. C., van der Leest, C., Djamin, R. S., Janssens, A. M., Hoogsteden, H. C., Sterenborg, H. J., Amelink, A., Aerts, J. G. Characterization of mediastinal lymph node physiology in vivo by optical spectroscopy during endoscopic ultrasound-guided fine needle aspiration. J Thorac Oncol 5, 981-987 (2010). [Crossref]
35. Li, A., Liu, J., Tanamai, W., Kwong, R., Cerussi, A. E., Tromberg, B. J. Assessing the spatial extent of breast tumor intrinsic optical contrast using ultrasound and diffuse optical spectroscopy. J Biomed Opt 13, 030504 (2008). [Crossref]
36. Chang, Y. H., Chuang, C. K., Ng, K. F., Liao, S. K. Coexistence of a hemorrhagic cyst and carcinoma in the prostate gland. Chang Gung Med J 28, 264-267 (2005).
37. Galosi, A. B., Montironi, R., Fabiani, A., Lacetera, V., Gallé, G., Muzzonigro, G. Cystic lesions of the prostate gland: an ultrasound classification with pathological correlation. J Urol 181, 647-657 (2009). [Crossref]

This article can be accessed at PubMed:
http://www.ncbi.nlm.nih.gov/pubmed/22066593

Received: June 3, 2011; Revised: July 31, 2011; Accepted: August 16, 2011

TCRT December 2011

category image
Volume 10
No.6 (505-634)
December 2011
ISSN 1533-0338

DOI: 10.7785/tcrt.2012.500219

Z. Jiang, Ph.D.1#
D. Piao, Ph.D.1*
K. E. Bartels, D.V.M./M.S.2
G. R. Holyoak, D.V.M./Ph.D.2
J. W. Ritchey, D.V.M./Ph.D.3
C. L. Ownby, Ph.D.4
K. Rock, D.V.M.2
G. Slobodov, M.D.5

1School of Electrical and Computer Engineering, Oklahoma State University, Stillwater, OK, USA
2Department of Veterinary Clinical Sciences, Oklahoma State University, Stillwater, OK, USA
3Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, OK, USA
4Microscopy Laboratory, Oklahoma State University, Stillwater, OK, USA
5Department of Urology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

#Currently in Lightlab Imaging Inc., Westford, MA, USA

*Corresponding author:
D. Piao, Ph.D.
E-mail: daqing.piao@okstate.edu

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