TCRT December 2005

Volume 4
No. 6 (p 583-712)
December 2005
ISSN 1533-0338

Drug Delivery

The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 Cells) from Pluronic P105 Micelles (p. 707-712)

This notes examines the mode of cell death of HL-60 cells exposed to 70 kHz and 1.3 W/cm² ultrasound in the presence of 1% Pluronic P105 and 1.67 μg/ml doxorubicin (Dox). The cells were ultrasonicated for 30, 60, and 120 minutes. They were then lysed, electrophorised, stained using propidium iodide, and their DNA profile captured using a fluorescent microscope. The gradual DNA damage observed and the comet tails captured after one and two hours of insonation suggest that the mode of cell killing is apoptosis.

Key words: Micellar drug delivery; Doxorubicin; Comet Assay; Ultrasound; and Apoptosis.

Ghaleb A. Husseini, Ph.D.^1,2,*
Kim L. O?Neill, Ph.D.³
William G. Pitt, Ph.D.¹

¹Department of Chemical Engineering
P.O. Box 26666
American University of Sharjah
Sharjah, United Arab Emirates
²Department of Chemical Engineering
Brigham Young University
Provo, Utah 84602, USA
³Department of Microbiology
Brigham Young University
Provo, Utah 84602, USA
*ghusseini@ausharjah.edu

Subscription is more cost effective than purchasing PDFs on-the-fly. Click here for details.

<< Go Back to TCRT December 2005

Ecommerce Solutions
Online Payment Solutions

Articles

TCRT December 2005

The Comet Assay to Determine the Mode of Cell Death for the Ultrasonic Delivery of Doxorubicin to Human Leukemia (HL-60 Cells) from Pluronic P105 Micelles (p. 707-712)

Purchase Downloadable Full-text PDF of Articles

Corporate Rate

University/Academic User